Background:  Protein kinases comprise a large group of encoded factors that regulate cellular processes by catalyzing the transfer of a phosphate group to a hydroxyl acceptor in serine, threonine or tyrosine residues. Myotonic dystrophy kinase-related Cdc42-binding (DMPK-like) kinases-a and b (MRCK-a, b) contain a cysteine-rich motif and a putative pleckstrin homology domain. MRCKs can phosphorylate nonmuscle myosin light chain and influences actin-myosin contractility. MRCK-a can phosphorylate and activate LIM kinases downstream of Cdc42, which leads to inactivation of ADF/cofilin and to Actin cytoskeletal reorganization. MRCK-a can also influence neurite outgrowth promoted by Cdc42 and Rac.

Description: Rabbit polyclonal to MRCK alpha

Immunogen: KLH conjugated synthetic peptide derived from MRCK alpha

Specificity:  ·Reacts with Human, Mouse, Dog and Rat.

·Isotype: IgG

Application:  ·Western blotting: 1/100-500. Predicted Mol wt: 186 kDa;

·Immunohistochemistry (Paraffin/frozen tissue section): 1/100-500;

·Immunocytochemistry/Immunofluorescence: 1/100;

·Immunoprecipitation: 1/50;

·ELISA: 1/500;

·Optimal working dilutions must be determined by the end user.