Background:  Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. ZKSCAN4 (Zinc finger protein with KRAB and SCAN domains 4), also known as ZNF307, is a 545 amino acid protein belonging to the Krüppel C2H2-type zinc-finger protein family. Localized to the nucleus, ZKSCAN4 is expressed in placenta, lung, kidney, brain and heart, as well as in embryonic (17-week) liver, brain, heart, small intestine and skeletal muscle. ZKSCAN4 functions as a transcription repressor and has been shown to suppress both p53 and p21 transcription. Overexpression of ZKSCAN4 results in the upregulation of p300 and MDM2 genes, suggesting that ZKSCAN4 suppresses the p53-p21 pathway by activating p300 and MDM2 expression, which then leads to the degradation of p53. Phosphorylation on Ser165 of human ZKSCAN4 occurs upon DNA damage, most likely by Atm or ATR.

Description: Rabbit polyclonal to ZNF307

Immunogen: KLH conjugated synthetic peptide derived from ZNF307

Specificity:  ·Reacts with Human, Mouse and Rat.

·Isotype: IgG

Application:  ·Western blotting: 1/100-500. Predicted Mol wt: 60 kDa;

·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;

·Immunocytochemistry/Immunofluorescence: 1/100;

·Immunoprecipitation: 1/50;

·ELISA: 1/500;

·Optimal working dilutions must be determined by the end user.