Background:  The HIRIP3 (HIRA interacting protein 3) locus encodes for a 556 amino acid protein that directly interacts with the HIRA histone chaperone. It also interacts weakly with core histones, Histone H2B and Histone H3. HIRIP3 is a heavily phosphorylated nuclear protein and it is found throughout the cell cycle. It is phosphorylated by casein kinase II. HIRIP3 may play a role in chromatin function and histone metabolism. A region (approximately 60 amino acids in length) at the C-terminus of HIRIP3 is highly conserved among vertebrates and it contains residues that are invariantly charged, polar, and hydrophobic. Two isoforms of HIRIP3 exists due to alternative splicing. Isoform 1 is predominately expressed in skeletal muscles and isoform 2 is expressed in the liver and the heart. Human HIRA homologs are thought to be responsible for the DiGeorge syndrome and related developmental disorders.

Description: Rabbit polyclonal to HIRIP3

Immunogen: KLH conjugated synthetic peptide derived from HIRIP3

Specificity:  ·Reacts with Human, Mouse and Rat.

·Isotype: IgG

Application:  ·Western blotting: 1/100-500. Predicted Mol wt: 62 kDa;

·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;

·Immunocytochemistry/Immunofluorescence: 1/100;

·Immunoprecipitation: 1/50;

·ELISA: 1/500;

·Optimal working dilutions must be determined by the end user.