Background:  CKAP2 (cytoskeleton associated protein 2) is localized to the cytoplasm of humans and is expressed in tissues, including thymus and testis. CKAP2 is also refered to as LB1, TMAP or se20-10, and is a 682 amino acid protein which is expressed as three isoforms. CKAP2 is utilized during mitosis and is involved in regulating functions of microtubules, cellular death and the cell cycle. Before mitosis, CKAP2 is expressed in the cell cycle between phases G1 and S, and accumulates between phases S and G2. During mitosis, when the anaphase promoting complex is activated, CKAP2 is degraded. The regulation of CKAP2 is essential for proper spindle functions and cytokinesis, and it is thought that CKAP2 function is mediated via phosphorylation and dephosphorylation. Upon activation of p53 by CKAP2, the G1 phase of the cell cycle is stopped, leading to cell death and apoptosis. Gastric tumors contain excessive amounts of CKAP2, which can lead to unregulated spindle functions may be involved in the development and progression of gastric cancer.

Description: Rabbit polyclonal to CKAP2

Immunogen: KLH conjugated synthetic peptide derived from CKAP2

Specificity:  ·Reacts with Human, Mouse and Rat.

·Isotype: IgG

Application:  ·Western blotting: 1/100-500. Predicted Mol wt: 77 kDa;

·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;

·Immunocytochemistry/Immunofluorescence: 1/100;

·Immunoprecipitation: 1/50;

·ELISA: 1/500;

·Optimal working dilutions must be determined by the end user.