Background:  Pygopus 2, also known as PYGO2, is a 406 amino acid protein that is the human homolog of the Drosophila Pygopus protein. Localized to the nucleus, Pygopus 2 contains one PHD finger that interacts with the homology domain of the Wnt signaling protein Bcl-9. This interaction joins Pygopus 2 with the β-catenin/TCF complex (a crucial complex in Wnt signaling), thereby allowing β-catenin to transcriptionally activate Wnt target genes. Association of Pygopus 2 with proteins involved in the Wnt signaling pathway is thought to regulate proper signal transduction, as absence of the Pygopus 2/β-catenin interaction may play a role in development of B cell malignancies. In addition, Pygopus 2 expression is upregulated in and required for the growth of breast cancer cells, suggesting a crucial role in carcinogenesis.

Description: Rabbit polyclonal to PYGO2

Immunogen: KLH conjugated synthetic peptide derived from PYGO2

Specificity:  ·Reacts with Human, Mouse and Rat.

·Isotype: IgG

Application:  ·Western blotting: 1/100-500. Predicted Mol wt: 41 kDa;

·Immunohistochemistry (Paraffin/frozen tissue section): 1/100-500;

·Immunocytochemistry: 1/100-200;

·ELISA: 1/500;

·Optimal working dilutions must be determined by the end user.