Background:  CD40 ligand (CD40L) is a 33 kDa type II membrane glycoprotein expressed mainly on the cell surface of activated T lymphocytes, but also exists as a soluble form extracellularly. CD40L is the ligand for CD40, a member of the TNF superfamily, which is expressed on the cell surface of B cells, macrophages/monocytes, dendritic cells, vascular endothelial cells, and epithelial cells. CD40L plays an important role in B cell proliferation, antibody class switching, modulation of apoptosis in the germinal center through interaction with B cells expressing CD40, and activation of CD4+ T cells. Mutation within the CD40L gene is linked to hyper IgM syndrome, an X linked immunodeficiency disease that is characterized by elevated level of serum IgM and decreased level of other isotypes.

Description: Rabbit polyclonal to CD40L, FITC conjugated

Immunogen: KLH conjugated synthetic peptide derived from CD40L

Specificity: Recognizes CD40L of Human, Mouse and Rat in Western blot and immunohistochemiscal staining.

Specificity:  ·Reacts with Human, Mouse and Rat.

·Isotype: IgG

Application:  ·Western blotting: 1/100-500. Predicted Mol wt: 29 kDa;

·Immunohistochemistry (Frozen/paraffin tissue section): 1/100-500;

·Immunocytochemistry: 1/100;

·Flow Cytometry: 1/25-100;

·Immunofluorescence: 1/25-100;

·Excitation spectrum: 490-495nm

·Emission spectrum: 520-530nm

·Optimal working dilutions must be determined by the end user.