Background:  GCIP (Grap2 (Gads) and cyclin D-interacting protein), also known as cyclin D-type binding-protein 1 (CCNDBP1), human homolog of maid (HHM) or DIP1, is a 360 amino acid cytoplasmic and nuclear protein belonging to the CCNDBP1 family. GCIP is interacts with cyclin D and Gads, a leukocyte-specific adaptor protein known to influence immune cell signaling. Suggested to regulate cell cycle progression, GCIP acts as a negative regulator of liver-specific gene expression and prevents Rb phosphorylation by inhibiting the Cdk4/cyclin D complex. GCIP expression is down-regulated in a number of tumors including those found in rectum, breast, prostate and colon, but up-regulated in hepatic cancers. GCIP is ubiquitously expressed and exists as at least four alternatively spliced isoforms whose expression likely increases during differentiation and can be induced by sodium butyrate.

Description: Rabbit polyclonal to GCIP

Immunogen: KLH conjugated synthetic peptide derived from GCIP

Specificity:  ·Reacts with Human, Mouse and Rat.

·Isotype: IgG

Application:  ·Western blotting: 1/100-500. Predicted Mol wt: 40 kDa;

·Immunohistochemistry (Paraffin/frozen tissue section): 1/100-200;

·Immunocytochemistry: 1/100;

·Immunoprecipitation: 1/50;

·ELISA: 1/500;

·Optimal working dilutions must be determined by the end user.