Background:  PLEKHM1 (Pleckstrin homology domain-containing family M member 1), also known as 162 kDa adapter protein, is a 779 amino acid protein that contains two Pleckstrin homology (PH) domains, which are found in proteins that are involved in intracellular signaling. PLEKHM1 is located in the cytoplasm where it is involved in osteoclast vesicular transport and is therefore an essential protein for bone resorption. Individuals having defects in PLEKHM1 are afflicted with osteopetrosis autosomal recessive type 6 (OPTB6), a rare genetic disease that is characterized by abnormally dense bone due to ineffective bone resorption. Specifically, it is likely that mutations in the PLEKHM1 gene affect endosomal acidification/maturation and TRACP exocytosis, which has implications on osteoclast-osteoblast cross-talk.

Description: Rabbit polyclonal to PLEKHM1

Immunogen: KLH conjugated synthetic peptide derived from PLEKHM1

Specificity:  ·Reacts with Human, Mouse and Rat.

·Isotype: IgG

Application:  ·Western blotting: 1/100-500. Predicted Mol wt: 117 kDa;

·Immunohistochemistry (Paraffin/frozen tissue section): 1/100-200;

·Immunocytochemistry: 1/100;

·Immunoprecipitation: 1/50;

·ELISA: 1/500;

·Optimal working dilutions must be determined by the end user.