Background:  Midline-2 (midline defect 2, tripartite motif-containing protein 1) is a 715 amino acid protein encoded by the human gene MID2. Midline-2 belongs to the TRIM/RBCC family and contains two B box-type zinc fingers, one B30.2/ SPRY domain, one COS domain, one Fibronectin type-III domain and one RINGtype zinc finger. Midline-2 is a cytoplasmic protein found as a homodimer or heterodimer with Midline-1. It also interacts with IGBP1 (lymphocyte signaling protein A4). Dimerization is mediated by the tripartite motif, RBCC (RING- and B box-type zinc fingers and coiled-coil domains), and microtubule association is dependent on the C-terminal B30.2 domain. Midline-2 is expressed at low levels in fetal kidney and lung, and in adult prostate, ovary and small intestine.

Description: Rabbit polyclonal to MID2

Immunogen: KLH conjugated synthetic peptide derived from MID2

Specificity:  ·Reacts with Human, Mouse and Rat.

·Isotype: IgG

Application:  ·Western blotting: 1/100-500. Predicted Mol wt: 83 kDa;

·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;

·Immunocytochemistry: 1/100;

·Immunoprecipitation: 1/50;

·ELISA: 1/500;

·Optimal working dilutions must be determined by the end user.