Background:  Ribosomes, the organelles that catalyze protein synthesis, are composed of a small subunit (40S) and a large subunit (60S) that consist of over 80 distinct ribosomal proteins. Mammalian ribosomal proteins are encoded by multigene families that contain processed pseudogenes and one functional intron-containing gene within their coding regions. Ribosomal Protein L5, also known as RPL5 or DBA6, is a 297 amino acid protein required for rRNA maturation that belongs to the Ribosomal Protein L18P family. A component of the 60S subunit, Ribosomal Protein L5 localizes to cytoplasm and nucleolus and binds 5S rRNA to form a stable complex termed 5S ribonucleoprotein particle (RNP), which is essential for nonribosome-associated cytoplasmic 5S rRNA transport into the nucleolus for ribosome assembly. Ribosomal Protein L5 defects may lead to Diamond-Blackfan anemia type 6 (DBA6), a congenital hypoplastic anemia that is characterized by erythroblastopenia and macrocytic anemia.

Description: Rabbit polyclonal to RPL5

Immunogen: KLH conjugated synthetic peptide derived from RPL5

Specificity:  ·Reacts with Human, Mouse and Rat.

·Isotype: IgG

Application:  ·Western blotting: 1/100-500. Predicted Mol wt: 34 kDa;

·Immunohistochemistry (Paraffin/frozen tissue section): 1/100-200;

·Immunocytochemistry: 1/100;

·Immunoprecipitation: 1/50;

·ELISA: 1/500;

·Optimal working dilutions must be determined by the end user.