Background:  The superfamily of GTP-binding proteins, of which Ras proteins are prototypes, has been implicated in a broad range of biological activities. Studies have identified a family of guanine nucleotide-releasing factors (GRFs) that activate Ras in mammalian cells and an “adapter” protein (Sem 5/GRB2) that appears to mediate the interaction of GRFs with activated receptor molecules. Subsequent to activation, Ras appears to interact with Raf, thereby activating the MAP kinase phosphorylation pathway. RasGRP is a guanyl nucleotide-releasing protein for Ras that contains two EF hand domains, which bind to calcium, and a diacylglycerol (DAG)-binding domain. RasGRP is expressed in the nervous system and lymphoid tissues and may link changes in DAG and calcium concentrations to Ras activation.

Description: Rabbit polyclonal to Rasgrp1

Immunogen: KLH conjugated synthetic peptide derived from Rasgrp1

Specificity:  ·Reacts with Human, Mouse and Rat.

·Isotype: IgG

Application:  ·Western blotting: 1/100-500. Predicted Mol wt: 90 kDa;

·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;

·Immunocytochemistry/Immunofluorescence: 1/100;

·Immunoprecipitation: 1/50;

·ELISA: 1/500;

·Optimal working dilutions must be determined by the end user.