Background:  The protein RB1CC1 (retinoblastoma 1 (RB1)-inducible coiled-coil 1) is a key regulator of the tumor-suppressor gene RB1. RB1CC1 is abundantly expressed in human musculoskeletal and cultured osteosarcoma cells in amounts closely correlated to RB1 expression. Both the RB1CC1 and RB1 genes are preferentially co-expressed and contribute to the maturation of human embryonic musculoskeletal cells. RB1CC1 is localized in the nucleus and may be a transcription factor indicated by the presence of its nuclear localization signal, leucine zipper motif and coiled-coil structure (a ubiquitous protein folding and assembly motif made of alpha-helices wrapped around each other to form a supercoil). The RB1CC1 gene has been identified in a screen for genes involved in multi-drug resistance to anticancer agents and is frequently mutated in breast cancer, showing characteristics of a tumor-suppressor gene. In mouse, Rb1cc1 protein is highly expressed in heart and testis, with lower levels detected in lung and spleen.

Description: Rabbit polyclonal to RB1CC1

Immunogen: KLH conjugated synthetic peptide derived from RB1CC1

Specificity:  ·Reacts with Human, Mouse and Rat.

·Isotype: IgG

Application:  ·Western blotting: 1/100-500. Predicted Mol wt: 38 kDa;

·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;

·Immunocytochemistry/Immunofluorescence: 1/100;

·Immunoprecipitation: 1/50;

·ELISA: 1/500;

·Optimal working dilutions must be determined by the end user.