Background:  Glucosylceramide synthase (GCS), also designated ceramide glucosyltransferase, belongs to the glycosyltransferase 2 family. It is a widely expressed integral membrane protein encoded by UGCG. The enzyme can be found in the plasma membrane of all eukaryotic cells, and a significant concentration of glucosylceramide synthase activity has been reported in the Golgi complex. Glucosylceramide synthase catalyzes the first glycosylation step in glycosphingolipid biosynthesis and functions as a glucosyltransferase and flippase in the transfer of glucose to ceramide. Glucosylceramide synthase operates in cell recognition, cell proliferation and differentiation, immune recognition and signal transduction. The regulation of ceramide levels through glucosylceramide synthase has been associated with the induction of apoptosis and notable research implicates this relationship with drug-induced apoptosis in a variety of cell types.

Description: Rabbit polyclonal to UGCG

Immunogen: KLH conjugated synthetic peptide derived from UGCG

Specificity:  ·Reacts with Human, Mouse and Rat.

·Isotype: IgG

Application:  ·Western blotting: 1/100-500. Predicted Mol wt: 45 kDa;

·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;

·Immunocytochemistry/Immunofluorescence: 1/100;

·Immunoprecipitation: 1/50;

·ELISA: 1/500;

·Optimal working dilutions must be determined by the end user.