Background: The c-Myc proto-oncogene plays a significant role in cell proliferation, differentiation, transformation and apoptosis. A novel c-Myc binding protein, MYCBP (also designated AMY-1), binds to the transactivation domain of c-Myc and stimulates the activation of E-box-dependent transcription. MYCBP translocates from the cytoplasm to the nucleus during S phase when increased expression of c-Myc occurs. MYCBP and AAT-1 (AMY-1-associating protein expressed in testis 1) have been shown to associate with AKAP 149 and AKAP 84 in mitochondria of somatic cells and sperm, which suggests a role for MYCBP and AAT-1 in spermatogenesis. Expression of the AAT-1 gene is regulated by two different promoters, which result in various isoforms. One promoter generates expression of the AAT-1, AAT-1å, AAT-1∫ and AAT-1© isoforms, which are specifically expressed in testis, while the other promoter generates AAT-1L, AAT-1M and AAT-1S, which are differentially expressed.

Description: Rabbit polyclonal to C3orf15

Immunogen: KLH conjugated synthetic peptide derived from C3orf15

Specificity:  ·Reacts with Human, Mouse and Rat.

·Isotype: IgG

Application:  ·Western blotting: 1/100-500. Predicted Mol wt: 71 kDa;

·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;

·Immunocytochemistry/Immunofluorescence: 1/100;

·Immunoprecipitation: 1/50;

·ELISA: 1/500;

·Optimal working dilutions must be determined by the end user.