Background: MDFIC is a cellular factor capable of modulating the expression of cellular and viral promoters. Its synthesis is controlled at the translational level by two different codons, an ATG and an upstream non-ATG translational initiator, allowing the production of two protein isoforms that present different subcellular localizations, p32 (isoform 2) being mainly distributed throughout the cytoplasm, whereas p40 (isoform 1) is targeted to the nucleolus. MDFIC is known to down-regulate Tat-dependent transcription of HIV-1 LTR by interacting with HIV-1 Tat and Rev proteins and impairing their nuclear import (possibly by rendering the NLS domains inaccessible to importin-beta) and stimulate activation of human T-cell leukaemia virus type I (HTLV-I) LTR. In human cells, MDFIC binds to the axin complex, resulting in an increase in the level of free beta-catenin; it affects axin regulation of the WNT and JNK signalling pathways.

Description: Rabbit polyclonal to MDFIC

Immunogen: KLH conjugated synthetic peptide derived from MDFIC

Specificity:  ·Reacts with Human, Mouse and Rat.

·Isotype: IgG

Application:  ·Western blotting: 1/100-500. Predicted Mol wt: 37 kDa;

·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;

·Immunocytochemistry/Immunofluorescence: 1/100;

·Immunoprecipitation: 1/50;

·ELISA: 1/500;

·Optimal working dilutions must be determined by the end user.