Background:  Belonging to the large family of nonpeptidic C-N hydrolases, nitrilases are enzymes that cleave nitriles and organic amides, resulting in carboxlic acid and ammonia. NIT1 (Nitrilase homolog 1) is a 327 amino acid protein that plays a role in cell growth and apoptosis. Loss of NIT1 expression leads to accelerated proliferation, increased cyclin D1 expression and resistance to DNA damage stress, whereas overexpression of NIT1 leads to caspase-dependent apoptosis. This evidence suggests that NIT1 functions as a tumor supressor. NIT1 is expressed in placenta, kidney, brain, liver, heart, pancreas and skeletal muscle where it is localized to both the cytoplasm and mitochondria. There are four isoforms of NIT1 that are produced as a result of alternative splicing events.

Description: Rabbit polyclonal to NIT1

Immunogen: KLH conjugated synthetic peptide derived from NIT1

Specificity:  ·Reacts with Human, Mouse, Dog and Rat.

·Isotype: IgG

Application:  ·Western blotting: 1/100-500. Predicted Mol wt: 35 kDa;

·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;

·Immunocytochemistry/Immunofluorescence: 1/100;

·Immunoprecipitation: 1/50;

·ELISA: 1/500;

·Optimal working dilutions must be determined by the end user.