Background:  Proline-rich proteins are often involved in protein-protein interactions and typically act as ligands for SH3, WW and EVH1 domains. PRR5 (Proline-rich protein 5), also known as Protein observed with Rictor-1 (Protor-1), is a 388 amino acid protein that is a subunit of mammalian target of rapamycin complex 2 (mTORC2), a multimeric kinase that is involved in the regulation of the actin cytoskeleton and phosphorylates Akt1, SGK and PKC å. Other proteins that make up mTORC2 include Rictor, MAPKAP-1, FRAP and LST8. PRR5 plays an important role in the modulation of platelet-derived growth factor signaling and in the regulation of PDGFR-∫ expression. Since the gene encoding PRR5 is located in a region that frequently undergoes loss of heterozygosity in breast cancer, it is likely that PRR5 is a tumor suppressor. There are four isoforms of PRR5 that are produced as a result of alternative splicing events.

Description: Rabbit polyclonal to PRR5

Immunogen: KLH conjugated synthetic peptide derived from PRR5

Specificity:  ·Reacts with Human, Mouse and Rat.

·Isotype: IgG

Application:  ·Western blotting: 1/100-500. Predicted Mol wt: 43 kDa;

·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;

·Immunocytochemistry: 1/100;

·Immunoprecipitation: 1/50;

·ELISA: 1/500;

·Optimal working dilutions must be determined by the end user.