Background:  Src is the human homolog of the v-Src gene of the Rous sarcoma virus, also known as avian sarcoma virus or ASV. Src is the first proto-oncogenic non-receptor tyrosine kinase characterized in human. By virtue of common structural motifs, the Src family is composed of nine members in vertebrates, including Src, Yes, Fgr, Frk, Fyn, Lyn, Hck, Lck and Blk. Src-family kinases transduce signals that control a variety of cellular processes, including proliferation, differentiation, motility and adhesion. Src-family kinases contain an amino terminal cell membrane anchor followed by an SH3 domain and an SH2 domain involved in modular association and activation, respectively. Human c-Yes is the cellular homolog of the Yamaguchi sarcoma virus oncogene, Yes1. The human c-Yes gene maps to chromosome 18 and encodes a 543 amino acid protein. c-Src and c-Yes kinases are more than 80% homologous outside of unique amino-termini. Their respective SH3 and SH2 domains are capable of directing specificity in substrate binding. Human c-Yes is subject to autophosphorylation at Tyr 537.

Description: Rabbit polyclonal to YES1

Immunogen: KLH conjugated synthetic peptide derived from YES1

Specificity:  ·Reacts with Human, Mouse, Pig, Dog and Rat.

·Isotype: IgG

Application:  ·Western blotting: 1/100-500. Predicted Mol wt: 61 kDa;

·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;

·Immunocytochemistry/Immunofluorescence: 1/100;

·Immunoprecipitation: 1/50;

·ELISA: 1/500;

·Optimal working dilutions must be determined by the end user.